Growth process of bilirubin and bile acid

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Abstract: Bile acid and bilirubin have a bile of animals, these two products have a greater potential for feed additives. This article introduces the production process of bile acid and bilirubin.

Keywords: bile acid; bilirubin; production process

Bile acid is the main active ingredient of bile, it is a series of sterols produced during cholesterol metabolism in animals. It is a mixture of compared components with multiple biological activity [1]. At present, there is frequent stress in the process of intensive breeding, and toxic and hazardous substances in feed raw materials and the pursuit of premature columns, increasing the burden of animal liver [2], so that the liver synthesizes bile acid and secretion bile The function is suppressed, thereby causing nutritional diarrhea, fatty liver, hepatitis, etc., resulting in an increase in the aquaculture breeding cost, forming unnecessary waste [3]. Bile acids are used as a feed additive to effectively alleviate the above problems. Shandong Longchang Animal Health Products Co., Ltd. has been committed to the study of bile acids and has achieved better results.

The study found that bilirubin is not only the metabolite in the body, which is still an endogenous strong antioxidant, anti-oxidation, anti-lipid peroxidation, protecting cells from damage And enhance the antioxidant capacity of vitamin C and vitamin E [4].

The two products of bile acids and bilirubin exist in the bile of animals. In order to better utilize resources, Shandong Longchang Animal Health Products Co., Ltd. is jointly produced by these two products, saving both production costs, and no resources.

1 bilirubin production process

1.1 Test reagent 1 ¡ë NaHSO3 aqueous solution, NaOH, chloroform, 3N hydrochloric acid solution, 95% ethanol, diethyl ether

1.2 Test material Shuanghui Pine bilirneam for the biochemical plant

1.3 Test instrument stainless steel knife, stainless steel reaction can, distillation bottle, suction filtration bottle

1.4.1 Heating bile: use Stainless steel knife or scissors will be placed in a stainless steel reaction tank while heating to 50 ¡ã C. 1.4.2 Saponified reaction: When the bile is heated to 50-57 ¡ã C, NaOH is slowly added, and the pH is adjusted to 11-12, continue to heat it, and until the boiling 3 minutes stop heating, Quickly cool below 20 ¡ã C. 1.4.3 Acidification treatment: 0.5-1 ¡ë NaHSO3 aqueous solution is added, and then the chloroform of about 50% in the total weight of the bile. After stirring, the aqueous hydrochloric acid solution is 45ml / kg, and the pH is adjusted to 4.5. The bile is divided into three layers. 1.4.4 Distillation: The layered chloroform slowly released, placed in a distillation bottle. The bile in the reaction tank was added to 40% chloroform, stirred at the same time, and then incorporated in a distillation bottle after 30 minutes.

1.4.5 Summary: The chloroform solution is distilled to finally see the biliary uulgin in the distillation bottle, add the same amount of 95% ethanol to evaporate, and the remaining liquid is carried out when the chloroform is noted. The filtration was filtered, then washed with diethyl ether, dried, and capable of product.

2 Bile Acid Production Process Test Reagent 10% NaOH solution, decolorizer, 3N hydrochloric acid solution 2.2 Test material Shuanghui Factory supplied pig bilir [ 123]

2.3 Test Instruments, tank, oxidation tank, acidized can, suction filtration bottle, drying box

Test method

2.4.1 Saponification process: will be blended The liquid is added to 10 times the volume of 10% by volume of NaOH solution, placed in the reaction tank, boiling for about 10 hours, stop heating, to be divided into layers, and exocerate the liquid.

2.4.2 Decolorization Process: After dissolving the sodium bile acids, heating to 80 ¡ã C to add the decolorizer. When the liquid color is close to yellow, it is placed in a tank after filtration.

2.4.3 Acidification Treatment: After the liquid after decolorization is reduced to room temperature, then the aqueous hydrochloric acid solution is added to 4.5 to adjust the pH. Such solids are basically precipitated.

2.4.4 Pumgmentation: The solid after the acid was filtered. Then, washed, the color to be solid is close to the white, dried, dry, and capable.

3 Conclusion

The content of bilirubin in bile is relatively low, batch of 1000 kilograms of raw materials The yield of bilirubin is almost 0.25 ¡ë, and the average purity of bilirubin is above 90%.

After extracting the bilixin, the content of bile acid is relatively high, the yield is about 50%, and the average purity is about 97%.

3 Precautions for Production Process

3.1 The bitter bold preferably with fresh, frozen or stored for too long to affect the cost rate.

3.2 bitter and bile avoided contact with iron.

3.3 Brushing containers, preferably use distilled water or deionized water.


[1] Zeng Rui, Li Li, Wang Quan, Liu Wanhan Feed Additive, 2002, (5): 1Jul 1987 (CA 1988, 108:221466c).

Guo Ying, Xie Jianping, Mo Zheng Ji.Mooji. New Progress in Pharmacology and Clinical Application of Hydrochloride [J]. Huaxi Medical,2004, 19 (4): 702 – 703. [4] Xu Shuyun. Pharmacological Experiment Method [M]. 3rd Edition. Beijing: People’s Medical Publishing House, 2001. 1376.

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