Vitamin production process

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1 Overview Vitamin is a class of biological growth development, different chemical structures, small molecular organic compounds, and humans cannot be synthesized from food, etc.. There is very little demand for human body, but when it is insufficient, the corresponding deficiency appears. There are 13 vitamins, depending on solubility, generally divided into two categories of water soluble and lipolysis vitamins. There are three methods for the production of major vitamins. In 2002, my country’s vitamin raw material was 82,000 tons, of which vitamin C exceeded 50,000 tons, which became the world’s largest raw material drug producer and exporter. The annual output of the vitamin preparation is 260-28 billion / piece / bottle, which is mainly based on tablets, injection and capsules. 2 Vitamin C Production Process Vitamin C (Vitamin C) is the world’s largest sales and the most wide range of vitamin products. At prnt, there is about 100,000 tons / year in the world world, and the global market has sales of 500 million US dollars. At prnt, industrially producing vitamin C a ts two-step fermentation, this method is the first study of the Shanghai Biotechnology Rarch Institute, Chin Academy of Sciences, in 1975. Fermentation method The production of vitamin C can be divided into fermentation, extraction and transformation. That is, first fermenting from D-sorbitol, extract vitamin C precursor 2-keto-L-ancient dragon acid, re-use chemical method into vitamin C. The first step of fermentation: ACETOBACTER Suboxydans is expanded in seeds, and the fermenter, seed and fermentation medium mainly include sorbitol, corn, yeast, and calcium carbonate, pH 5.0 ~ 5.2. The alcohol concentration is controlled at 24 to 27%, the culture temperature is 29 to 30 ¡ã C, and after the fermentation is completed, the fermentation liquid is subjected to a low temperature, and the second step fermentor is transferred to the raw material. D-sorbitol conversion of L-samylate of 98%. The second step fermentation: oxidized glucuronacillus (Gluconobacter oxydans, fungi) and a mixed culture of Bacillus Megaterium, apronium. The fermenter of vitamin C is 100 m3 or more, thin long, no mechanically stirred, and gas rising stirring. The seed and fermentation medium are similar, mainly L-sorbitos, corn, urea, calcium carbonate, potassium phosphate, etc., the pH is 7.0. Large and small bacteria were expanded by secondary seeds. In the fermenter containing the first step fermentation liquid, a large amount of sterile air was transferred at 29 to 30 ¡ã C, and the incubation of about 72 h was completed, L-sorbitose production 2- The conversion of keto-L-¹ÅÁú has reached 70 to 85%. Separation of 2-keto-L-¹ÅÁú Acid: After two-step fermentation method, only about 8% of 2-keto-L-¹Ålonic acid containing only 8% left, and residual mycelium, protein and Impurities such as suspended solid particles, often using heating precipitation, chemical coagulating method, and ultrafiltration. The conventional process is to heat the precipitation method. The fermentation liquid is adjusted by a 732 hydrogen type ion exchange resin column after standing.The equidistant, and heated to solidify the protein, then separated by mycelial, protein, and particles with high speed centrifuge, the liquid is again passed through the cation exchange column, acidified into an aqueous solution of 2-keto-L-¹ÅÁú Acid, concentrated crystallization 2-keto-L-ancient dragon acid. 2-keto-L-Cologne acid chemical transformation: converting vitamin C precursor 2-keto-L-¹Åalic acid into vitamin C, often used alkali transformation. 2-keto-L-¹ÅÁú Acid uses concentrated sulfate to catalyzate methyl 2-ketoside-L-¹ÅÁú ionate, add NaHCO3 to form vitamin C sodium salt, and acidified from hydrogen-free ion exchange resin to obtain vitamin C . The crude product was purified by a vitamin C finished product. 3 Vitamin B2 Production Process Vitamin B2 (Vitamin B2) is also known as ribofln, and there are about L0 nuclear plasma manufacturers in China. At prnt, there is widely used microbial fermentation method in China to produce vitamin B2. It can produce microorganisms of vitamin B2 have bacteria, fungi and mold, industrial production, mainly based on AshbothHerecium ashbyii as production. The industrial fermentation of vitamin B2 is generally a secondary fermentation, and the fermentation liquid is first precipitated by separation and purification. In fermentation medium, vegetable oil, glucose, glucogene or rice or the like as the main carbon source, and the promotion of soybean oil on the production of soybean oil in the vegetable oil is most significant, the organic nitrogen source is dominated by protein, bone glue, fish powder, corn, and inorganic salt. NaCl, K2HPO4, MGSO4. The variation of seed expansion culture and fermentation is relatively high, and the ve are generally at 1.0, and the can press 0.05 MPa, and the agitation power requireme are relatively high. The optimum growth temperature of the auxiliary vast yeast is from 28 to 30 ¡ã C, and the seed culture is 34 to 38 hours. After the fermentation culture is started after 40 h, the pH of the fermentation liquid is controlled at 5.4 to 6.2, fermentation The cycle is 150 ~ 160h. The yield of vitamin B2 is around 50 g / L. Separation and purification of vitamin B2: After the fermentation tray is heated, then a yellow blood salt, zinc sulfate precipitate protein, filtrate, filtrate, pH 1.5 ~ 2.0 acidification, stand for 20 to 40 hours, precipitate, and quately , An ammonium nitrate, oxidation and extraction, and the oxidized fluid was crystallized, filtered, and the moistocyst was dried at 80 ¡ã C for 20 hours to obtain a vitamin B2 finished product.

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