Vitamin production process

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1. Vitamin is a class of biological growth development, different chemical structures, small molecular organic compounds, and humans cannot be synthesized from food, etc.. There is very little demand for human body, but when it is insufficient, the corresponding deficiency appears. There are 13 vitamins, depending on solubility, generally divided into two categories of water soluble and lipolysis vitamins. There are three methods for the production of major vitamins.
In 2002, my country’s vitamin raw material production reached 82,000 tons, of which vitamin C exceeded 50,000 tons, which became the world’s largest raw material producer and exporting country. The annual output of the vitamin preparation is 260-28 billion / piece / bottle, which is mainly based on tablets, injection and capsules.
2 Vitamin C Production Process

Vitamin C) is the world’s largest production and the widest range of vitamin products. At present, there is about 100,000 tons / year in the world world, and the global market has sales of 500 million US dollars. At present, industrially producing vitamin C adopts two-step fermentation, this method is the first study of the Shanghai Biotechnology Research Institute, Chinese Academy of Sciences, in 1975. Fermentation method The production of vitamin C can be divided into fermentation, extraction and transformation. That is, first fermenting from D-sorbitol, extract vitamin C precursor 2-keto-L-ancient dragon acid, re-use chemical method into vitamin C.

The first fermentation: ACETOBACTER Suboxydans passes through seeds to expand cultivation, access fermentants, seeds and fermentation medium mainly include sorbitol, corn, yeast, calcium carbonate, PH 5.0 ~ 5.2 . The alcohol concentration is controlled at 24 to 27%, the culture temperature is 29 to 30 ¡ã C, and after the fermentation is completed, the fermentation liquid is subjected to a low temperature, and the second step fermentor is transferred to the raw material. D-sorbitol conversion of L-samylate of 98%.
Second step fermentation: Gluconobacter Oxydans, Specia and Mixed Culture of Bacillus (Bacillus MegaTerium, Activity). The fermenter of vitamin C is 100 m3 or more, thin long, no mechanically stirred, and gas rising stirring. The seed and fermentation medium are similar, mainly L-sorbitos, corn, urea, calcium carbonate, potassium phosphate, etc., the pH is 7.0. Large and small bacteria were expanded by secondary seeds. In the fermenter containing the first step fermentation liquid, a large amount of sterile air was transferred at 29 to 30 ¡ã C, and the incubation of about 72 h was completed, L-sorbitose production 2- The conversion of keto-L-¹ÅÁú has reached 70 to 85%.
Separation of 2-keto-L-¹ÅÁú Acid: After two step fermentation method, only 2-keto-L-¹Å ¹Å ketic acid containing ratio of about 8%, and residual mycelium Impurities such as protein and suspended solid particles, often using a heated precipitation method, a chemical coagulant method, and a super filtration method. Traditional process is heating precipitateMethod, the fermentation broth is deprecated by the 732 hydrogen type ion exchange resin column after deposition, and the pH is adjusted to the protein or the like, and the protein is solidified, then the hyphae, protein and particles are separated by high-speed centrifuge, and the liquid is once again passed. The cation exchange column is acidified into aq.
2-keto-L-Cologne acid chemical transformation: transforming vitamin C precursor 2-keto-L-¹ÅÁú acid into vitamin C, often used alkali transformation. 2-keto-L-¹ÅÁú Acid uses concentrated sulfate to catalyzate methyl 2-ketoside-L-¹ÅÁú ionate, add NaHCO3 to form vitamin C sodium salt, and acidified from hydrogen-free ion exchange resin to obtain vitamin C . The crude product was purified by a vitamin C finished product.
3 Vitamin B2 Production Process
Vitamin B2 (Vitamin B2) is also known as riboflavin, about about L0 nuclear plasma producers in China. At present, there is widely used microbial fermentation method in China to produce vitamin B2. It can produce microorganisms of vitamin B2 have bacteria, fungi and mold, industrial production, mainly based on AshbothHerecium ashbyii as production. The industrial fermentation of vitamin B2 is generally a secondary fermentation, and the fermentation liquid is first precipitated by separation and purification.

In fermentation medium, vegetable oil, glucose, glucogene or rice or the like as the main carbon source, the promotion of soybean oil on the production of soybean oil in the vegetable oil, the organic nitrogen source is mainly used by protein, bone glue, fish powder, corn, mainly, The inorganic salt has NaCl, K2HPO4, MgSO4. The variation of seed expansion culture and fermentation is relatively high, and the vents are generally at 1.0, and the can press 0.05 MPa, and the agitation power requirements are relatively high. The optimum growth temperature of the auxiliary vast yeast is from 28 to 30 ¡ã C, and the seed culture is 34 to 38 hours. After the fermentation culture is started after 40 h, the pH of the fermentation liquid is controlled at 5.4 to 6.2, fermentation The cycle is 150 ~ 160h. The yield of vitamin B2 is around 50 g / L.

Separation and purification of vitamin B2: After the fermentation liquid is heated, then a yellow blood salt, zinc sulfate precipitate protein is added, and the filtrate is filtered. Soluble, adding ammonium nitrate, oxidation, and oxidized fluid crystallization, filtered, wet crystalline is dried over 80 ¡ã C for 20 h, resulting in a vitamin B2 finished product.

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